<D0926> Insect DNA Kit 昆虫DNA提取试剂盒

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‍昆虫DNA提取试剂盒

Insect DNA Kit

产品编号	产品名称	包装规格	目录价
D0926-00	昆虫DNA提取试剂盒	5T	135
D0926-01	昆虫DNA提取试剂盒	50T	810
D0926-02	昆虫DNA提取试剂盒	200T	3150

产品介绍

The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.

参数

FEATURES	SPECIFICATIONS
Starting Amount	50 mg
Starting Material	Insect tissue
Yield	100 µg
Elution Volume	50–100 µL
Technology	HiBind® DNA Mini Column
Processing Mode	Manual
Throughput	1–24

组分

Product	D0926-00	D0926-01	D0926-02
Purifications	5	50	200
HiBind® DNA Mini Columns	5	50	200
2 mL Collection Tubes	10	100	400
CTL Buffer	2 mL	20 mL	80 mL
CBL Buffer	3 mL	30 mL	120 mL
HBC Buffer	3 mL	25 mL	80 mL
Proteinase K Solution	150 µL	1.4 mL	4 × 1.4 mL
RNase A	12 µL	120 µL	450 µL
DNA Wash Buffer	1.5 mL	15 mL	3 × 20 mL
Elution Buffer	1 mL	20 mL	50 mL
User Manual	✓	✓	✓

流程

Lyse
Extract with chloroform
Bind
Wash
Dry
Elute

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