血液DNA大提试剂盒
Blood DNA Maxi Kit
产品编号 | 产品名称 | 包装规格 | 目录价 |
D2492-01 | 血液DNA大提试剂盒 | 5T | 450 |
D2492-02 | 血液DNA大提试剂盒 | 20T | 1710 |
D2492-03 | 血液DNA大提试剂盒 | 50T | 3600 |
产品介绍
The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.
参数
FEATURES | SPECIFICATIONS |
Starting Amount | Up to 20 mL |
Starting Material | Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat |
Yield | 2 mg |
Elution Volume | 2000 µL |
Technology | HiBind® DNA Maxi column |
Processing Mode | Manual, centrifugation/vaccuum |
Throughput | 1–24 |
组分
Product | D2492-03 |
Preparations | 50 |
HiBind® DNA Maxi Columns | 50 |
50 mL Collection Tubes | 50 |
TL Buffer | 375 mL |
BL Buffer | 600 mL |
HBC Buffer | 200 mL |
DNA Wash Buffer | 2 × 200 mL |
RNase A | 1.1 mL |
Proteinase K Solution | 13 mL |
Elution Buffer | 500 mL |
User Manual | ✓ |
流程
Lysis
Adjust Binding Conditions
Bind
Wash
Elute