bioGenous™ 人结直肠类器官培养试剂盒是一种专为成年干细胞来源的人结肠类器官设计的无血清培养基。结肠上皮的自我更新由隐窝内干细胞及其前体细胞的增殖驱动。在原代培养基中生长的人结肠类器官主要包含 LGR5+ 干细胞、处于增殖状态的过渡扩增细胞（TA）、早期肠上皮细胞（CDX2+、Villi+）。而在维持培养基中培养的类器官则含有成熟的肠上皮细胞（CDX2+、MUC2+）、杯状细胞（MUC2+）、M细胞（GP2+）、肠内分泌细胞以及少量微绒毛细胞（tuft cells）。

Product Description

bioGenous™ Human Colonic Organoid Kit is a serum-free culture medium for human colonic organoids derived from adult stem cells. Self-renewal of colonic epithelium is driven by the proliferation of stem cells and their progenitors located in the crypts. Human colonic organoids grown in primary culture medium consisted of LGR5+ stem cells, cycling transit-amplifying (TA) cells, early enterocytes (CDX2+, Villi+). While human colonic organoids grown in maintenance medium contain mature enterocytes (CDX2+, MUC2+) and goblet cells (MUC2+), M cells (GP2+) and enteroendocrine cells, as well as a low number of tuft cells. Human colonic organoids display hallmarks of the colonic epithelium in terms of architecture and cell type composition, therefore hold great promise for studies of human colonic development and disease. Human colonic organoids may also have applications in regenerative biology through ex vivo expansion of the colonic epithelium.