德国MN NucleoBond Xtra Midi EF无内毒素质粒中提试剂盒
德国MN NucleoBond Xtra Midi EF无内毒素质粒中提试剂盒
货号:740420.50
规格:50 preps
品牌:德国MN
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA
Application | Endotoxin-free plasmid DNA isolation |
Selling unit | 10 Prep(s), 50 Prep(s) |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Midi prep |
Handling | Gravity flow |
Lysate clarification | Column filter |
Automated use | Yes, can be automated on the Andrew+ pipetting robot |
Sample material | Bacteria, E. coli |
Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
Vector size | < 300 kbp |
Typical yield | 500 µg |
Theoretical binding capacity | 1000 µg |
Typical purity A260/A280 | 1.8−1.95 |
Endotoxin level | ≤ 0.05 EU/µg DNA |
Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
Preparation time | 85 min/prep |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 39 Month(s) |
Hazardous material | Yes |