德国MN Nucleobond Xtra Midi Plus 超快速转染级质粒中提试剂盒
货号:740412.50
规格:50 preps
品牌:德国MN
从大肠杆菌中提取高拷贝/低拷贝质粒。新一代阴离子交换柱-短时间,高产量,转染用质粒。
特点:
节约60%的时间,增加100%的产量
新一代阴离子交换–超快,高产量的转染级别的质粒DNA
优化设计的柱子:制备时间约为30 分钟(Midi prep);35 分钟(Maxi prep)
包含柱过滤器:高过虑流速,清除裂解液中不溶物的同时可向柱子加样
改进的硅材料:标准的产量250μg-1000μg
专li的阴离子交换技术得到转染级别的质粒DNA
NucleoBond(R)Xtra Plus kits 包含NucleoBond(R) Finalizers;加速了DNA的沉淀
2nd generation of anion exchanger for fast purification of plasmid DNA, NucleoBond Finalizer included
Application | Transfection-grade plasmid DNA isolation |
Selling unit | 10 Prep(s), 50 Prep(s) |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Midi prep |
Handling | Gravity flow |
Lysate clarification | Column filter |
Automated use | No |
Sample material | Bacteria, E. coli |
Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
Vector size | 2−50 kbp |
Typical yield | 500 µg |
Theoretical binding capacity | 1000 µg |
Typical purity A260/A280 | 1.8−1.95 |
Endotoxin level | 1−10 EU/µg DNA |
Isopropanol precipitation | NucleoBond Finalizer |
Preparation time | 30 min/prep |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 39 Month(s) |
Hazardous material | Yes |