<D3396> Tissue DNA Kit 组织DNA提取试剂盒

分享到:
The E.Z.N.A. Tissue DNA Kit offers a versatile and cost-effective method for isolating DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc.

组织DNA提取试剂盒

Tissue DNA Kit


产品编号

产品名称

包装规格

目录价

D3396-00

组织DNA提取试剂盒     

5T

126

D3396-01

组织DNA提取试剂盒  

50T

720

D3396-02

组织DNA提取试剂盒  

200T

2610


产品介绍

The E.Z.N.A. Tissue DNA Kit offers a versatile and cost-effective method for isolating DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with Silica Mini Spin Column technology into four quick lyse, bind, wash, and elute steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation and the use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis, and restriction enzyme digestion.


参数

FEATURES

SPECIFICATIONS

Downstream Applications

PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion

Starting Material

Tissues, cultured cells, mouse tail snips, paraffin-embedded tissues, whole blood, body fluids, buccal swabs

Starting Amount

30 mg, or 5 × 10 cultured cells

Elution Volume

100–200 µL

DNA Binding Technology

Silica mini spin column

Throughput

1–24

Processing Time

< 20 min (post-lysis)


组分

Product

D3396-00

D3396-01

D3396-02

D3396-03

Purifications

5

50

200

600

HiBind® DNA Mini Columns

5

50

200

600

2 mL Collection Tubes

10

100

400

1200

BL Buffer

5 mL

20 mL

60 mL

2 × 100 mL

TL Buffer

5 mL

20 mL

60 mL

135 mL

HBC Buffer

5 mL

25 mL

80 mL

250 mL

DNA Wash Buffer

2.5 mL

25 mL

3 × 25 mL

200 mL

Elution Buffer

15 mL

30 mL

2 × 60 mL

300 mL

Proteinase K Solution

150 µL

1.5 mL

6 mL

18 mL

User Manual


流程

  • Lyse

  • Adjust Binding Conditions

  • Bind

  • Wash 3X

  • Dry

  • Elute