Vivacell 超低内毒素DMEM/F-12(含谷氨酰胺、HEPES)

栏目:Vivacell 液体培养基
产品名称:DMEM/F-12, HEPES With L-Glutamine

货号:C3131-0500

规格:500ml

品牌:Vivacell

产品名称:DMEM/F-12, HEPES With L-Glutamine

货号:C3131-0500

规格:500ml

价格:询价


Product Description

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham(DMEM/F12 1:1 mixture) with L-Glutamine and HEPES was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.

It is super low endotoxin SLETM grade,Endotoxin≤0.01EU/ml by LAL method. The production process and quality management system meet the requirements of cGMP and has passed ISO13485 certification.

It does not contain trace elements. Users are advised to review the literature for recommendations regarding medium supplementation and suitable cell lines.


【Composition】

IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate154.500
Copper sulphate pentahydrate0.0013

Disodium hydrogen phosphate anhydrous

71.020

Ferric nitrate nonahydrate0.050
Ferrous sulphate heptahydrate0.417
Magnesium chloride hexahydrate61.200
Magnesium sulphate anhydrous48.840
Potassium chloride311.800
Sodium chloride6996.000
Sodium dihydrogen phosphate anhydrous54.300
Zinc sulphate heptahydrate0.432

AMINO ACIDS
Glycine18.750

L-Alanine

4.450
L-Arginine hydrochloride147.500

L-Asparagine monohydrate

7.500

L-Aspartic acid

6.650

L-Cysteine dihydrochloride

17.560

L-Cystine hydrochloride monohydrate

31.290
L-Glutamic acid7.350
L-Glutamine365.000
L-Histidine hydrochloride monohydrate31.480
L-Isoleucine54.470
L-Leucine59.050
L-Lysine hydrochloride91.250
L-Methionine17.240
L-Phenylalanine35.480
L-Proline17.250
L-Serine26.250
L-Threonine53.450
L-Tryptophan9.020
L-Tyrosine Disodium Salt48.100

L-Valine

52.850

VITAMINS
Choline chloride8.980

D-Biotin

0.0035
D-Pantothenic acid2.240
Folic acid2.660
Niacinamide2.020
Pyridoxal hydrochloride2.000
Pyridoxine hydrochloride0.031
Riboflavin0.219
Thiamine hydrochloride2.170
Vitamin B120.680

myo-Inositol

12.600

OTHERS

D-Glucose

3151.000

DL-Thioctic Acid

0.105
HEPES buffer3574.500
Hypoxanthine2.400
Linoleic acid0.042
Phenol red Sodium Salt8.630
Putrescine hydrochloride0.081
Sodium pyruvate110.000
Thymidine0.365
Sodium bicarbonate1200.000


【Storage and Stability】

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham(DMEM/F12 1:1 mixture) with L-Glutamine and HEPES should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.

As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:

(a). Color Change;

(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments;

(c). Insolubility;

(d). And/or decrease in expected performance parameters.

Any material described above should not be used and therefore discarded.

 

【Procedure】

1.Take a bottle from the defined storage conditions at 2-8°C and read the label.

2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.

3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.

4.Antibiotics may be added if desired.

 

【Quality Control】

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham(DMEM/F12 1:1 mixture) with L-Glutamine and HEPES is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.

 

【Precaution and Disclaimer】

For research use only, not for clinical diagnosis and treatment.