His Protein Pull Down Kit
产品名称 | 规格 | 货号 | 目录价 |
His Protein Pull Down Kit | 5T | BK0055-t | 800 |
His Protein Pull Down Kit | 25T | BK0055-01 | 3000 |
产品介绍:
His Protein Pull Down Kit是一款能够高效完成标签诱饵蛋白与靶蛋白共沉降的试剂盒。其包含高性能Co Smart Beads 6FF,能够高特异性的捕获带有 6×、9×、12×His 标签的诱饵蛋白。另外试剂盒内经过优化的缓冲液,为蛋白互作实验提供了最佳的反应条件,增强了蛋白互作实验的稳定性,更有效的去除实验背景。Co Smart Beads 6FF 是一种新型 IMAC 填料,螯合有非常牢固的Co 离子,拥有更为广泛的适配性,试剂耐受情况见表1。
表1:Co Smart Beads 6FF试剂耐受情况
试剂种类 | 耐受时间 |
0.01M HCI, 0.01M NaOH | 1 week |
10mM EDTA, 1M NaOH, 5mM DTT, 5mM TCEP,20mM β-mercapyoethanol, 6M Gu-HCI | 24h |
500mM imidazole,100mM EDTA | 2h |
30% isopropanol | 20min |
本产品可广泛应用于大肠杆菌裂解物、细胞裂解物、细胞分泌上清等样品中的蛋白互作验证,具体组分见表 2。
表2. His Protein Pull Down Kit产品组分
组分名称 | 规格(5T) | 规格(25T) |
Co Smart Beads 6FF | 0.15ml | 0.75ml |
Lysis Buffer A | 5ml | 25ml |
Lysis Buffer B(1000x) | 0.1ml | 0.5ml |
Wash Buffer Enhanced | 2.5ml | 12.5ml |
Balance/Wash Buffer(10x) | 10ml | 50ml |
Elution Buffer | 0.4ml | 2ml |
Pull-down Column Accessory | 10套 | 50套 |